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MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz Language: English | Size: 770 MB | Duration: 1h 32m DNA cloning and synthetic biology What you'll learn The development of genetic engineering and synthetic biology DNA cloning techniques and plasmid design Uses for genetic engineered systems DNA modification enzymes (DNA polymerases, restriction enzymes, nucleases, phosphatases/kinases, recombinases, and more) Requirements Basic knowledge of DNA, RNA, and proteins Description
Designing, building, and testing genetic designs has created the interdisciplinary field of synthetic biology that rationally designs biological systems, most often using DNA assembly. Biological systems are fascinating because they store information that is autocatalytic and self-replicating. This course is an introduction to the techniques of genetic engineering, tips for their application, and discussion of use-cases. The first module will review key concepts of molecular biology and biological system information storage, the eras of biological research and major technological breakthroughs, and how genetic elements have come to be defined and now abstracted. The second module focuses on the DNA modification techniques widely in use, the enzymes and other components, and design principals are covered. The final module looks at the applications of these techniques for genetically modified organisms. The future of distributed synthetic biology is also highlighted. Example experiments are included at the conclusion of the course to help see the new knowledge in action.
Genetic engineering has gone from a advanced laboratories to elementary classrooms as the techniques become more widely known and materials available. To build plasmids, genetic circuits, and engineered organisms a knowledge of DNA modification techniques is needed. These tools are available to a resourceful biohacker and will be familiar at the end of the course.
Who this course is for: Biohackers looking to get a broad view of genetic engineering Biotechnology students interested in DNA cloning Biotechnology professionals needing coverage of the foundations of synthetic biology
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FILE LIST
Filename
Size
~Get Your Files Here !/01 - What is a gene/001 Promo.mp4
16.3 MB
~Get Your Files Here !/01 - What is a gene/001 Promo_en.vtt
1.1 KB
~Get Your Files Here !/01 - What is a gene/002 Class 1. From DNA to proteins.mp4
57 MB
~Get Your Files Here !/01 - What is a gene/002 Class 1. From DNA to proteins_en.vtt
7.2 KB
~Get Your Files Here !/01 - What is a gene/003 Class 2. Genetic engineering to synthetic biology.mp4
63.4 MB
~Get Your Files Here !/01 - What is a gene/003 Class 2. Genetic engineering to synthetic biology_en.vtt
6.5 KB
~Get Your Files Here !/01 - What is a gene/004 Class 3. Genetic elements.mp4
66.2 MB
~Get Your Files Here !/01 - What is a gene/004 Class 3. Genetic elements_en.vtt
8.6 KB
~Get Your Files Here !/02 - DNA cloning techniques/001 DNA modification.mp4
44.8 MB
~Get Your Files Here !/02 - DNA cloning techniques/001 DNA modification_en.vtt
6.3 KB
~Get Your Files Here !/02 - DNA cloning techniques/002 Polymerase chain reaction (PCR).mp4
86.4 MB
~Get Your Files Here !/02 - DNA cloning techniques/002 Polymerase chain reaction (PCR)_en.vtt
10.6 KB
~Get Your Files Here !/02 - DNA cloning techniques/003 Restriction enzymes and ligation.mp4
79.8 MB
~Get Your Files Here !/02 - DNA cloning techniques/003 Restriction enzymes and ligation_en.vtt
8.3 KB
~Get Your Files Here !/02 - DNA cloning techniques/004 Isothermal assembly.mp4
32.1 MB
~Get Your Files Here !/02 - DNA cloning techniques/004 Isothermal assembly_en.vtt
4.1 KB
~Get Your Files Here !/02 - DNA cloning techniques/005 In vivo assembly and recombinases.mp4
81.9 MB
~Get Your Files Here !/02 - DNA cloning techniques/005 In vivo assembly and recombinases_en.vtt
10.1 KB
~Get Your Files Here !/03 - Types of genetic engineering/001 Disruption of function.mp4
66.9 MB
~Get Your Files Here !/03 - Types of genetic engineering/001 Disruption of function_en.vtt
8.5 KB
~Get Your Files Here !/03 - Types of genetic engineering/002 Metabolic engineering.mp4
105.5 MB
~Get Your Files Here !/03 - Types of genetic engineering/002 Metabolic engineering_en.vtt
10.7 KB
~Get Your Files Here !/03 - Types of genetic engineering/003 Synthetic biology.mp4
70.5 MB
~Get Your Files Here !/03 - Types of genetic engineering/003 Synthetic biology_en.vtt
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