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Language: English | Size: 1.04 GB | Duration: 2h 33m
CRISPR/Cas Systems, Applications and Project Designs
What you'll learn
You will learn genetic treatment methods in target diseases with CRISPR-Cas approaches.
Design CRISPR Cas project
Design guide RNA, Decision of best Cas system
Base editing, Prime Editing, Transposase and Knockout systems
Requirements
Participating in the RadiChal competition
Description
The CRISPR revolution has begun and shows no signs of slowing down. This system, which is the key to prokaryotic adaptive immunity, has proven to be particularly under the influence of genome engineering. CRISPR/Cas provides flexibility as well as easily amplifying the signal and regulating the signal level, which is beyond the capabilities of previous systems. Prokaryotes have long exploited CRISPR/Cas as a powerful defense strategy against virus-derived invaders, and eventually, this system turns out to be useful only for research applications.Just when we thought that genome engineering could not get any further, the CRISPR (clustered regularly spaced short palindromic repeats)/Cas9 system emerged. It is an important component of the bacterial immune system, allowing bacteria to recognize and destroy phages. In genome engineering applications, guide RNA (gRNA) sequence homology targets the Cas9 endonuclease to a specific locus—where the double helix is broken. Like ZFNs and TALENs, CRISPR/Cas9 uses HDR, but using RNA to determine editing makes the system more sensitive and malleable, as well as less expensive and time-consuming. For this reason, CRISPR/Cas9 has proven invaluable for high-throughput genome engineering.With a variety of robust and effective genome engineering methods at our fingertips, we have entered the Golden Age of genome engineering. Current studies have focused on developing these techniques in the hope that they will be clinically useful to provide desired target loci (or loci), high specificity, and efficacy. The most exciting thing we've found at Addgene is the cratization of genome engineering that has enabled and will continue to enable researchers around the world to use these tools. To learn all about CRISPR, you must complete the course.CRISPR/Cas Gene Editing Training Programi. What is CRISPR-Cas?ii. What are CRISPR-Cas Systems?iii. CRISPR Genetic Therapy Applicationsiv. Planning Your CRISPR Projectv. Guide RNA Design & Benchlingvi. Confirmation of your Gene Editing
Overview
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FILE LIST
Filename
Size
~Get Your Files Here !/1 - Introduction What is CRISPRCas/1 - Introduction What is CRISPRCas English.vtt
23.3 KB
~Get Your Files Here !/1 - Introduction What is CRISPRCas/1 - Introduction What is CRISPRCas.mp4
206.7 MB
~Get Your Files Here !/2 - Chapter 2 What are CRISPRCas Systems/2 - chapter 2 English.vtt
20 KB
~Get Your Files Here !/2 - Chapter 2 What are CRISPRCas Systems/2 - chapter 2.mp4
134.1 MB
~Get Your Files Here !/2 - Chapter 2 What are CRISPRCas Systems/3 - chapter 2 continue English.vtt
30.4 KB
~Get Your Files Here !/2 - Chapter 2 What are CRISPRCas Systems/3 - chapter 2 continue.mp4
250.8 MB
~Get Your Files Here !/3 - chapter 3 CRISPRCas Genetic Therapy Applications/4 - chapter 3 English.vtt
17.6 KB
~Get Your Files Here !/3 - chapter 3 CRISPRCas Genetic Therapy Applications/4 - chapter 3.mp4
146.7 MB
~Get Your Files Here !/4 - chapter 4 Designing a CRISPRCas Gene Editing Project/5 - chapter 4 English.vtt
34.9 KB
~Get Your Files Here !/4 - chapter 4 Designing a CRISPRCas Gene Editing Project/5 - chapter 4.mp4
241.6 MB
~Get Your Files Here !/5 - chapter 5 CRISPRCas Gene Editing Confirmation Methods/6 - chapter 5 English.vtt
13.1 KB
~Get Your Files Here !/5 - chapter 5 CRISPRCas Gene Editing Confirmation Methods/6 - chapter 5.mp4
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